Genetic diversity of Fritillaria persica (Liliaceae) in Iran based on ISSRs

Fritillaria persica belonging to monotypic subgen. Theresia is important for its medicinal and ornamental value. Its distribution in Iran covers the Zagros chain, and its populations and individuals show variations in perianth size and color. Our previous investigation on chloroplast nucleotide sequences showed that this variation had no application in defining subspecies. Various investigations (eg. Badfar-Chaleshtori et al., 2012, Li et al., 2009, Li et al., 2011) showed the usefulness of ISSRs in studying genetic diversity of species in this genus. In this work, genetic relationships among 72 individuals of F. persica from natural populations through Zagros Chain was studied using 10 ISSRs separated on PAGE. Results showed that all markers produced polymorphic bands and showing relationships between individuals and populations on dendrogram. Results are presented as a table of polymorphism of markers and plots of clustering and ordination of 72 samples. Results also show the application of ISSRs for vast evaluation of these markers for different evolutionary studies and showing important areas with high genetic diversity

Keywords:

Fritillaria persica , ISSRs , Genetic diversity , Zagros , Iran