Designing and fabrication of electrochemical aptasensor based on a novel MWCNTs@Au nanocomposite for bisphenol A detection

Bisphenol A (BPA) is a harmful endocrine disruption which is extensively used in production of beverage bottles, baby bottles, children s toys, and also the inner surface coating of cans used for food and the like [1]. Because of the negative effects of BPA on the environment and human health, the detection of BPA is urgently needed both for food safety concerns, and environmental monitoring. In recent years, the proposed sensing techniques based on electrochemical transducer especially, aptasensors have made the BPA detection simpler, faster, and more economic [2]. In this study, we provided an electrochemical aptasensor using anti-BPA aptamer as the recognition probe and MWCNTs@Au nanocomposite as an immobilization platform for BPA detection. The anti-BPA aptamer was immobilized on a glassy carbon electrode modified with MWCNTs@Au nanocomposite. The interaction between the aptamer and the BPA molecules was monitored by cyclic voltammetry (CV) in the electrochemical probe. The conformation of aptamer showed a change after BPA binding, triggering a decrease in the electron transfer of Fe(CN)6 3-/4- on the electrode surface. The observed decline was detectable as a function of the presence of BPA. The calibration graph was obtained in the concentration range of 0.1-8 nM with limit of detection 0.03 nM for BPA.