New Hydrazide Palladium Complexes, Synthesis, Characterization and biological studies

Four new complexes of palladium with general formula trans-[Pd(L)2(OAc)2] (E,F)and [Pd(L)(-OAc)]2(OAc)2 (G,H) (L= Benzhydrazide and 2-Furic hydrazide) have been synthesized and characterized with several methods including FT-IR, 1HNMR, elemental analysis, UV-Vis spectroscopy, then the fluorescence quenching reactions of complexes with bovine serum albumin (BSA) in pH 7.20 Tris–HCl buffer solution were studied and the interaction of them with calf thymus deoxyribonucleic acid (DNA) have been studied by electronic absorption titration, fluorescence and viscosity measurement. Also in order to determination of protein binding site of mentioned complexes, site‐competitive replacement experiments with specific site markers including Eosin-Y, Ibuprofen and Digoxin have been done. By the analysis of fluorescence spectrum and fluorescence intensity, it was showed that these complexes have an ability to quench the intrinsic fluorescence of BSA. Further our complexes showed the ability of interaction with binding site I of BSA. In addition, fluorescence resonance energy transfer (FRET) have been calculated. Spectroscopic measurements data showed the groove binding of them to DNA. Also DNA cleavage and cytotoxicity studies of synthesized complexes have been done