Arezoo Najafi, - Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran
Mohammad Reza Yaftian - Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran
Bahman Farajmand - Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran

Pramipexole is a medication using in treatment of Parkinson s disease and restless legs syndrome. High performance liquid chromatography (HPLC) is frequently used for determination of pramipexole in different real samples [1,2]. Ion mobility spectrometry (IMS) is a fast, sensitive and inexpensive technique used in pharmaceutical analysis [3]. This method can be applied as an alternative method for HPLC.Analysis of pramipexole in the complex real samples need to a proper sample-preparation method. Microextraction methods can be considered as convenient and reliable methods for reaching this goal. This communication intends to describe a dispersive liquid phase microextraction method, assisted with a homogenizer, for extraction-preconcentartion of pramipexole from aqueous samples followed by its determination by ion mobility spectrometry (IMS). Figure 1 shows an ion mobility spectrum of pramipexole. Different parameters affecting the efficiency of the method like solvent type, stirring rate, ionic strength and stirring time have been evaluated and optimized. Analytical performances of the method were evaluated under optimized conditions. The linearity range of the method was found to be 50-1000 ng/mL with a limit of detection of 18 ng/mL. The method demonstrated adequate precision with relative standard deviations (RSDs) lower than 7.1%. Finally, method was successfully used for determination of spiked pramipexole in different real samples including urine, plasma and pharmaceutical samples. The relative recoveries of pramipexole ranged from 82 to 107 %.