Sensing platform for adefovir based on molecularly imprinted polymer- coated carbon nanocomposite

In this paper, a novel electrochemical sensor with sensitive and selective binding sites for adefovir detection is reported, which is based on a carbon nanocomposite glassy carbon electrode (C/GCE) modified with molecularly imprinted of poly(o-phenylenediamine) templated with adefovir. The surface feature of electrode was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and field emission scanning electron microscopy (FESEM). Several important parameters controlling the performance of electrode were investigated and optimized using response surface methodology (RSM) [2,3]. Under optimal conditions, the changes in DPV peak currents of the redox probe, hexacyanoferrate, were linear to adefovir concentrations in the range from 1.2 to 21 μmol L-2, with the detection limit of 1.11 μmol L-2 (S/N = 4). The biological applicability of the developed sensor has been delineated by the determination of adefovir in human plasma and urine samples using the standard addition method. The proposed sensor exhibited high degree of selectivity for adefovir in comparison to other structurally similar biomolecules present in biological samples, along with long term stability, good reproducibility and excellent capacity of regeneration of molecular recognition sites.