Colorimetric Detection of D-Penicillamine Based on the Aggregation of Gold Nanoparticles

We report herein the development of a highly sensitive colorimetric method for detection of DPenicillamine using citrate capped gold nanoparticles (Au-NPs) . This assay relies upon the distance-dependent of gold nanoparticles surface plasmonic resonance band of gold nanoparticles. By replacing the thiol-containg chelator drug, D- Penicillamine, with citrate on the gold Nanoparticles surface , a new peak appearing at a longer wavelength intensifies and shifts further to the red from the original peak position which depends on ionic strength, DPenicillamine and gold nanoparticles. The ratio intensities of absorbance at longer wavelength (630 nm) to original wavelength (521 nm) allows the simple quantitative spectrophotometric detection of D-Penicillamine in pharmaceutical and biological samples.

Keywords:

Gold nanopartcles , Surface Plasmon Resonance Band , D- Penicillamine , Colorimetric Detection