Next Generation PGD: New challenge in Embryos transfer

Infertility is relatively common, and expected to increase along with reproductive-age couples delay child birth. Despite the success and widespread use of Assisted Reproductive Technologies (ART), there are concerns regarding its effect. Although it is estimated that more than 6 million children have been born over the past three decades by using ART, there are some studies addressing the adverse ART outcomes and suggested an increased risk of chromosome abnormalities, low birth weight and preterm delivery.Since the late 1980s, Preimplantation Genetic Diagnosis (PGD) has provided valuable help to select good-quality embryos for transfer and improve implantation, consequently ART successful rate. Recently, a variety of molecular techniques have been developed and validated to PGD that have revolutionized this approach. Great advances have been made by generating sufficient embryo DNA for both array and sequencing based methods.In clinical practice, you might encounter complex and complicated situations. Sometimes, you encounter couples that although both have a diploid normal karyotype, but in multiple ART cycles, they have not a single euploid blastomere. Using advanced molecular PGD, you detected triple X or extra copy of X q in affected embryos without a predominantly another aneuploidy. Also, you may find submicroscopic autosomal chromosomal aberrations with unknown prognostic significance.What do you do if these couples don’t want benefit from surrogacy embryo This is a major challenge, particularly when prognosis a sub-chromosomal aberration is not clear. Do you suggest transfer these affected embryos Do you trust embryo self-corrective mechanisms Recent evidence suggests mis-segregated chromosomes were encapsulated following extrusion from the embryo. Do you expect zygotic gene rescue cure genetic condition outcome

Keywords:

Next-Generation-Sequencing-PGD , Molecular PGD , Zygotic Gene Rescue , Embryo Self-Corrective Mechanisms , ART Failure