Changes in the Molecular Weight and pH Isoelectric of proteins in a malignant tumor of human Glioma

Proteins with known pH isoelectric points (pI) and native molecular weight (MW), but pI is more specific to MW. Isoelectric point is mean. The pH at which a particular molecule carries no net electrical charge or is electrically neutral in the statistical Previous research shows that even conserved proteins subjected to strong selection constraints follow the global trend in the pi distribution. Gliomas are the most frequent primary brain tumors and include a variety of different histological tumor type and malignancy grades. Glioma tumors are derived from glial cells and are sub-divided into astrocytoma, oligodendroglioma and oligoastrocytoma. We extracted proteins of tumor and normal brain tissues and then evaluated the protein purity by Bradford test. In this study, we separated proteins by Two-Dimensional Gel Electrophoresis method and the spots were then analyzed and compared using statistical data and specific software, SDS-PAGE gels were scanned using scanner Densitometer GS-800 (BioRad) scanner at 600 dpi in tagged image file format (TIFF). Spots were identified by pH isoelectric, molecular weights and data banks. The molecular weight and isoelectric pH of each of the proteins (G protein beta subunit, GBN1 protein, mutant beta-actin and alpha-tubulin) compared to control independently. Our results showed that the pI and MW of proteins in the tumor tissue has changed. The distribution of the isoelectric point of proteins in a protein is universal for all organisms.

Keywords:

pH Isoelectric , Molecular Weight , Protein , Glioma