Nafiseh Khosravi Dehaghi - Evidence-based Phytotherapy & Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran.Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran
Khadijeh Mahmoodi - Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran

Borago officinalis (Boraginaceae) is a medicinal plant with different components such as Phenolic compounds, flavonoides, rosmarinic acid, pyrrolizidine alkaloids, etc. It is used as anticonvulsant, bronchodilator and vasodilator. Echium amoenum (Boraginaceae) is a medicinal plant that is used in anxiety and depression. Different compounds such as Pyrrolizidine alkaloids, rosmarinic acid were isolated from its extracts. The aim of this study is elucidating the antioxidant activity of Borago officinalis and Echium amoenum different fractions by DPPH assay. Total phenolic contents of these plants fractions were determined spectrophotometrically using Folin-Ciocalteau reagent. This study showed that Borago officinalis ethyl acetate fraction (32.2112.23 µg GAE/mg dry Fraction) and Echium amoenum methanolic fraction (126.23 ± 2.62 µg GAE/mg dry Fraction) contained highest total phenolic content. IC52 of Borago officinalis ethyl acetate fraction (63.43 12.23 µg/ml) and Echium amoenum methanolic fraction (32.2412.51 µg/ml) were the best. This study showed that phenolic compounds may be the main sponsors to the antioxidant activity. These plants could be effective in antioxidant purposes.

Borago officinalis, Echium amoenum, Total Phenol content, DPPH assay