مقایسه اثر لیزات پلاکتی و پلاسمای غنی از پلاکت گوساله به عنوان محرک رشد بر میزان رشد و قابلیت حیات رده های مختلف سلولی

Background: Cell therapy and cell culture have received much attention in recent decades. Suitable cell growth requires growth supplements such as fetal bovine serum (FBS). FBS is component rich in nutrients, growth factors and supplementary compounds. However, FBS utilization has some limitations including mass production. Therefore, finding alternatives with the same growth promoting effects is inevitable. OBJECTIVES: This study was designed to compare the effect of bovine platelet lysate (PL) and PRP on different cell lines as a cost effective and available alternative for FBS. METHODS: Three conventional cell lines were investigated. Protein pattern of PL and platelet rich plasma (PRP) in comparison to FBS was determined using SDS page electrophoresis, and MTT and plating efficiency of cell lines in presence of PL and PRP were evaluated. RESULTS: The results demonstrated that platelet rich plasma and platelet lysate could increase cells’ viability similar to FBS. These results were significant in comparison with control group. CONCLUSIONS: It can be concluded that platelet lysate could be a valuable candidate to replace FBS in cell culture techniques, however, more studies should be done to understand its exact efficacy.

Keywords:

Cattle , fetal bovine serum , fetal bovine serum alternatives , platelet lysate , platelet rich plasma