جداسازی و مطالعه ویژگیهای فنوتیپی و مولکولی گونههای آئروموناسهای متحرک عامل سپتیسمی هموراژیک باکتریایی در برخی مزارع مبتلای کپورماهیان پرورشی کشور
Publish place: Iranian Journal of Veterinary Medicine، Vol: 10، Issue: 3
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJVM-10-3_007
تاریخ نمایه سازی: 12 تیر 1398
Abstract:
Background: Motile Aeromonas species cause heavymortalities in carp farms during spring and summer in Iran. Objectives: The aim of this study was to detect phenotypicand genotypic characterization of motile Aeromonas species isolated fromdiseased carps in some northern and southern provinces of Iran. Methods: A number of 40 samples from 22 fish farms werecollected. The identified motile Aeromonas species were sequenced andphylogenetic tree was drawn by MEGA6 using UPGMA analysis. Results: A number of 19 bacterial isolates wereidentified as motile Aeromonas sp. by biochemical tests, and the DNAsegments of 16S rRNA gene of all these strains gave 1200 bp after running on 1%agarose electrophorus gel. Also, the sequencing results showed that thebacterial samples were determined as A. hydrophila and A. veroniibiovar veronii. Conclusions: Phylogenetic analysis revealed that motile Aeromonasstrains in this study were separated in two clusters and four genogroups with high similarities
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Authors
Mahdi Soltani
۱Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Seyedeh Mahsa Moghimi
PhD Candidate, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran
Hoseinali Ebrahimzade Mousavi
Full professor, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran
Kazem Abdi
Assistant professor, Aquatic animal health expert, Directorate of health and control of aquatic animal diseases, Iranian veterinary organization
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