sahar aminkar - Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Abdolali Shojaeiyan - Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Sajad Rashidi Monfared - Department of Plant Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Mahdi Ayyari - Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.

Dioscin, HPLC, quantitative determination, Secondary metabolites, steroidal saponin