Blue Diode and Red He-Ne Lasers Affect the Growth of Anthocyanin Producing Suspension Cells of Apple

Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped test tubes were exposed to short-term laser radiation for 20 min in a rotary shaker. The fresh cell weight (FCW), dry cell weight (DCW), cell volume after sedimentation (CVS), cell number (CN) and cell viability (CV) as criteria of cell growth were recorded at 0, 4, 8, 12 days during cell culture. The cell growth was negatively affected in response to BDL and RHNL compared to control and darkness, respectively. The FCW and DCW was enhanced by BDL whereas were not affected by RHNL. Also, only 30.4 mWcm-2 intensity of BDL could increase CVS in RFA cells. Changes in CN were not displayed by RHNL and BDL. BDL more than RHNL decreased CV. Cell death rates observed due to BDL and RHNL were 40.42% and 33.67%, respectively. All these results showed that these lasers had diverse effects on FRA cell growth, however, these cells were more sensitive to BDL than RHNL especially in higher intensities, presumably because of its damage to cell membrane leading to cell death.

Keywords:

Cell growth , Cell suspension culture , Apple , Red He-Ne laser , Blue diode laser