Comparison of the Cytotoxic Effects of Nanoparticulate and Microparticulate Calcium Sodium Phosphosilicate Mouthwashes on Human Gingival Fibroblasts: an in-vitro Study

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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JR_JDMT-8-3_005

تاریخ نمایه سازی: 18 تیر 1398

Abstract:

Introduction:  This study sought to assess the cytotoxic effects of nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes on human gingival fibroblasts (HGFs). Methods: This in vitro study was conducted on HGFs isolated and cultured in a 48-well plate containing standard culture medium for evaluation of four concentrations of the two mouthwashes at two time points plus a positive and a negative control group. The HGFs were exposed to 0.001, 0.01, 0.1 and 1 mg/mL concentrations of mouthwashes for 1 and 24 hours. Positive and negative control cells were exposed to saline and distilled water, respectively. Cell viability was assessed using the methyl thiazolyl tetrazolium (MTT) assay, and the number of viable cells was counted in triplicate using a cell counter after transfection (trypsin-EDTA 0.25%, 20 minutes) and exposure to trypan blue. The optical density (OD) values were read by ELISA reader and analyzed using the Kruskal-Wallis test. Results: Number of viable cells was not significantly different between the two mouthwashes at the two time points (P> 0.05). At one hour, number of viable cells was higher in the nanoparticulate group while the number of viable cells in the microparticulate group was higher at 24 hours.  Conclusion: Nanoparticulate and microparticulate calcium sodium phosphosilicate mouthwashes have no cytotoxicity against HGFs. Cytotoxicity of the nanoparticulate mouthwash was less than that of the microparticulate mouthwash. Also, increased proliferation of fibroblasts was noted over time in both groups of mouthwashes.    

Authors

Shabnam Aghayan

Assistant Professor, Department of Periodontology, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Roya Assadi

Private Dentist, Faculty of Dentistry, Tehran dental Branch, Islamic Azad University, Tehran, Iran

Abdolmajid Bayandori Moghaddam

Assistant Professor of Chemistry, School of Engineering Science, College of Engineering, University of Tehran, Tehran, Iran

Ehsan Seyedjafari

Associate Professor, Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

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