Seyedeh Faezeh Taghizadeh - Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Horticulture and Landscape Engineering, Mashhad, Iran
Gholamhossein Davarynejad - Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Horticulture and Landscape Engineering, Mashhad, Iran
javad asili - Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mashhad University of Medical Sciences, Iran.
Seyed Hossein Nemati - Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Horticulture and Landscape Engineering, Mashhad, Iran

Objective: In this study, the levels and antioxidant activities of some secondary metabolites isolated from five pistachio (Pistacia vera)cultivars collected from four different geographical regions of Iran, were studied. Materials and Methods: Total phenolic compounds levels were determined by Folin-Ciocalteu method. Total flavonoid content was determined as AlCl3 complex and expressed as mg of quercetin equivalents (QE)/g dry extract and total proantocyanidins content was expressed as mg of catechin equivalents (CA)/g dry extract. In order to evaluated the antioxidant activity of the compounds, DPPH and FRAP assays were used. Results: The highest level of total phenols (156.42 mg GA/g DE), total flavonoids (130.94 mg QE/g DE) and total proantocyanidins (152.816 mg CA/g DE) were obtained in Akbari cultivar from Rafsanjan, followed by Badami-e-sefid and Ahmad aghaei. The lowest amount of total phenolic content (TPC), total flavonoid content (TFC) and total proanthocyanidin content (TPrAC) were found in Badami-e-sefid from Feizabad (128.140 mg GA/g DE, 93.176 mg QE/g DE and 118.870 mg CA/g DE, respectively). Also, a positive correlation (r2=0.9834) was found between antioxidant activity and total phenolic compounds. Conclusion: Pistachio increased their phytochemical compounds to contrast with abiotic stress. Our data could be useful for introducing special characteristics to the plants, and can be considered when planning a new breeding program or choosing a specific cultivar for a particular use.

Antioxidant activity, DPPH, Ferric reducing, Pistacia vera