Prevalence of Toxoplasma gondii Antibodies and DNA in Iranian HIV Patients
Publish place: IRANIAN JOURNAL of PATHOLOGY، Vol: 14، Issue: 1
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
View: 415
This Paper With 8 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
این Paper در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJP-14-1_009
تاریخ نمایه سازی: 23 تیر 1398
Abstract:
Background & Objective: Toxoplasma gondii infection has public health importance and can lead to serious diseases in immunosuppressed patients, such as HIV cases. Appropriate control of T. gondii infection in HIV patients requires information about the prevalence of T. gondii antibodies and DNA in different population. In this study, we aimed to determine the prevalence of Toxoplasma gondii antibodies and DNA in HIV patients in Tehran, Iran.Methods: A total of 149 HIV patients from the Iranian Research Center for HIV/AIDS, Tehran, Iran were enrolled in the study. Anti-Toxoplasma IgG and IgM were detected by ELISA and T. gondii DNA was evaluated by PCR and quantitative real-time PCR. IgG positive samples were also assessed for their avidity. Results: Anti-Toxoplasma IgG and IgM were positive in 46.3% and 2.7% of cases respectively. 92.7% of our patients showed past infection and 4.3% revealed recently acquired toxoplasmosis based on their IgG avidity test. T. gondii DNA was not detected by PCR but real-time PCR results showed DNA in 4.7% of total patients and 13.1% of the IgG seropositive cases.Conclusion: Our findings indicated that latent toxoplasmosis was relatively prevalent in our study population, but new T. gondii infection had low prevalence. Almost half of our patients were IgG negative and at risk of acquiring toxoplasma infection. Low copy numbers of DNA were detected in 4.7% of the cases without any clinical manifestation. Therefore, detection and monitoring of anti-Toxoplasma antibodies and DNA in HIV patients is substantial to estimate the risk of reactivation and new infection.
Keywords:
Authors
Anahita Bavand
MSc, Dept. of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
Arezoo Aghakhani
Anatomical & Clinical Pathologist, Dept. of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
Minoo Mohraz
Infectious Diseases Specialist, Iranian Research Center for HIV/AIDS, Tehran, Iran
Mohammad Banifazl
Pediatrician, Iranian Society for Support of Patients with Infectious Disease, Tehran, Iran