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Paper
Title

The Comparison Between Microwave and Autoclave as Antigen Retrieval Methods for Immunohistochemical Detection of CD15 and CD30 in Hodgkin’s Lymphoma

فصلنامه آسیب شناسی ایران، دوره: 13، شماره: 4
Year: 1397
COI: JR_IJP-13-4_001
Language: EnglishView: 178
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Authors

Farid Kosari - Dept. of Pathology, Tehran University of Medical Sciences, Tehran, Iran
Fatemeh Ghaffari - Pathology Laboratory, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Abstract:

Background and Objective: Hodgkin s lymphoma is a potentially curable hematologic malignancy with difficulty in its diagnosis especially in atypical cases even in expert hands. Today, immunohistochemistry plays a significant role in the diagnosis of it especially applying the anti-CD15 and anti-CD30 antibodies. The negativity of CD15 can be reduced by antigen retrieval for methods. In this study, the effect of autoclave was compared with microwave as heating sources of antigen retrieval in immunohistochemical staining.Methods: Sections prepared from 50 formalin-fixed paraffin-embedded tissue blocks of Classic Hodgkin s lymphomas stained for CD15 and CD30 using autoclave and microwave, were randomly and blindly reviewed by an expert hematopathologist, mostly focusing on Reed-Sternberg cells; the intensities were scored from 0 to +4 and analyzed by SPSS software.Results: Fifty eight percent of patients were male. The mean age was 32 years (range: 7 to 77). Nodular sclerosis was the most prevalent subtype. CD15 positivity in microwave treatment was 92% compared to 50% in autoclave. Negative CD30 decreased from 20% in autoclave to 2% in microwave. Intensity of staining in both markers was at least +1 greater in microwave treatment. No background staining was seen in microwave method.Conclusion: There was bimodal age distribution in Hodgkin s lymphoma patients with the predominant male and Nodular Sclerosis as the most common type. Comparing autoclave and microwave, higher rate of the positivity was detected using microwave treatment, especially in CD15 staining. Improvement in staining intensity was also noticeable in both markers. There was no background or non-specific staining in microwave method. No disturbance of cells or nuclear morphology was also reported. PMID: 30774676      PMCID: PMC6358563

Keywords:

Paper COI Code

This Paper COI Code is JR_IJP-13-4_001. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/896962/

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Kosari, Farid and Ghaffari, Fatemeh,1397,The Comparison Between Microwave and Autoclave as Antigen Retrieval Methods for Immunohistochemical Detection of CD15 and CD30 in Hodgkin’s Lymphoma,https://civilica.com/doc/896962

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  • Konkay K, Paul TR, Uppin SG, Rao DR. Hodgkin lymphoma: ...
  • Gobbi PG, Ferreri AJ, Ponzoni M, Levis A. Hodgkin lymphoma. Crit ...
  • Piccaluga PP, Agostinelli C, Gazzola A, Tripodo C, Bacci F, ...
  • Listinsky CM. A practical approach to the diagnosis of Hodgkin ...
  • Ohana-Malka O, Benharroch D, Isakov N, Prinsloo I, Shubinsky G, ...
  • Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, ...
  • Larsson L-I. Immunocytochemistry: Theory and Practice. CRC Press; 1988. [Google Scholar] ...
  • Mason JT, O’Leary TJ. Effects of formaldehyde fixation on protein ...
  • Taylor CR, Cote RJ. Immunomicroscopy: a diagnostic tool for the surgical ...
  • Huang SN. Immunohistochemical demonstration of hepatitis B core and surface ...
  • Fraenkel-Conrat H, Brandon BA, Olcott HS. the reaction of formaldehyde ...
  • Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, ...
  • Wu CY, Chen ST, Chiou SH, Wang KT. Specific peptide-bond ...
  • Roge R, Nielsen S, Vyberg M. Carb-3 Is the Superior ...
  • Fields P, Wrench D. Hodgkin Iymphoma. Medicine. 2013;41(5):290–294. [Google Scholar] ...
  • Jaffe ES, Harris NL, Vardiman J, Arber DA, Campo E. Hematopathology ...
  • Pileri SA, Ascani S, Leoncini L, Sabattini E, Zinzani PL, ...
  • Arici DS, Aker H, Güngör M. Utility of CD15, CD30 ...
  • Wasielewski RV, Mengel M, Fischer R, Hansmann ML, Hubner K, ...
  • Charalambous C, Singh N, Isaacson PG. Immunohistochemical analysis of Hodgkin’s ...
  • Cuevas EC, Bateman AC, Wilkins BS, Johnson PA, Williams JH, ...
  • Hunt NC, Attanoos R, Jasani B. High temperature antigen retrieval ...

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Type of center: علوم پزشکی
Paper count: 9,440
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