w.s Darwish - Food Control Department, Faculty of Veterinary Medicine, Zagazig University ۴۴۵۱۹, Egypt
A.S Atia - Department of Veterinary Hygiene, Faculty of Veterinary Medicine, Zagazig University ۴۴۵۱۹, Egypt
W.R El-Ghareeb - Food Control Department, Faculty of Veterinary Medicine, Zagazig University ۴۴۵۱۹, Egypt.Department of Veterinary Public Health and Animal Husbandry, College of Veterinary Medicine, King Faisal University, Saudi Arabia
A.E Elhelaly - Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt,. Center for Emerging Infectious Diseases, School of Medicine, Gifu University, Gifu, Japan

Background: Shiga toxin-producing Escherichia coli (STEC) are group of E. coli causing bloody diarrhea. The goal of this survey was to determine the prevalence of multidrug resistant shiga toxin-producing E. coli in cattle meat and its contact surfaces. Methods: Swab samples (n=120) were randomly collected from meat and contact surface of butchery shops in Sharkia province, Egypt. Prevalence of E. coli was examined using culture, biochemical, and serological methods. Identification of shiga toxin-encoding genes (stx1 and stx2) in the E. coli serotypes was done using multiplex polymerase chain reaction. Screening of multidrug resistance profile was done using the disk-diffusionmethod. Data were analyzed using JMP statistical package, SAS Institute Inc., Cary, NC. Results: The prevalence rates of E. coli in the chuck, round, masseter muscles, cuttingboards, walls, and floors were 20, 10, 30, 50, 40, and 60%, respectively. Among the isolates, E. coli O111:H4 and E. coli O26:H11 harbored the two mentioned genes. E. coli O86 and E. coli O114:H21 harbored only stx1; while E. coli O55:H7 encoded only stx2. Just E. coli O124 had no express of stx1 and stx2. The isolated E. coli serovars showed amultidrug resistance profile. Conclusion: Considering the results of this study, strict hygienic procedures should be followed to avoid or reduce carcass cross-contamination. In addition, proper handling and efficient cooking of meat are highly recommended by consumers to reduce the risk of human exposure to STEC.

Escherichia coli,Meat,Drug Resistance, Microbial,Polymerase Chain Reaction,Egypt,