The Potential Diagnostic Utility of Co-expression of Ki-67 and P53 in the Renal Biopsy in Pediatric Lupus Nephritis

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ACPLMED20_053

تاریخ نمایه سازی: 29 تیر 1398

Abstract:

Background The proliferative activity as long as apoptosis has been suggested to play a role in the pathogenesis of Lupus Nephritis (LN). The aim of the study was to investigate the co-expression of Ki-67 triggered marked proliferation and P53 induced apoptosis in renal biopsy of childhood Lupus Nephritis (cLN) and to compare co-expression of proliferative and apoptotic indices between different subgroups and clinicopathologic patterns of renal disease. MethodsRenal biopsy specimens of 33 children with Lupus Nephritis (LN) and 10 healthy subjects were retrospectively evaluated. The type of Lupus Nephritis and activity and chronicity indices were determined. Ki-67 and P53 immunostaining were performed. The co-expression Ki-67 and P53 were compared among different subgroups of Lupus Nephritis and correlated with disease activity index, serum creatinine, proteinuria, anticardiolipin antibodies, and complement levels. Histopathological examination of lupus nephritis classified based on the International Society of Nephrology/Renal Pathology. Histological LN activity was measured by the NIH Activity Index (NIH-AI). Results In comparison with the healthy control group, co-expression of Ki-67and P53 was greater in cLN (particularly in classes II, III and IV) than in normal renal tissue. Co-expression of Ki-67and P53 shows a positive correlation with subclasses II, III and IV of lupus nephritis (P<0.02) and LN activity index. (P < 0.03) Moreover, the positive correlation was found between co-expression of Ki-67 and P53 with ESR (P<0.02), D-dimer (P<0.03), serum creatinine (P<0.03), proteinuria (P<0.04) and Anticardiolipin Ab (P<0.05) significantly. Unexpectedly, the adverse correlation between co-expression of Ki-67 and P53 with serum C3 (P < 0.02) and C4 complement (P<0.03) was significant. ConclusionOur data showed that the co-expression of Ki-67 induced marked proliferation and P53 induced apoptosis in proliferative and active phases of cLN could reflect a valuable marker for treatment and remission in cLN patients before reaching towards the end stage of renal disease.

Authors

Mahtab Rahbar

Iran university of medical sciences

Maryam Rahbar

Tehran university of medical sciences

Nyousha Mardanpour

Iran university of medical sciences

Sourena Mardanpour

۳. Santa monica college .CA ,LA,USA