DH Alamdari - Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
M Aghasizadeh-Sharbaf - Department of Molecular Medicine, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
M Mohadjerani - Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
GA Ferns - Metabolic syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

BACKGROUND AND OBJECTIVE: Diabetes mellitus (DM) is a major health problem with an increasing global prevalence. It is usually associated with an imbalance between pro-oxidant mechanisms and antioxidant defenses, contributing to oxidative-stress, and this leads to an increased susceptibility to endothelial dysfunction, atherosclerosis, insulin-resistance and impaired-pancreatic β-cell function. We have assessed the Prooxidant-antioxidant balance (PAB) and anti-hemolytic effect of Thuja orientalis L using the PAB assay and the analysis of hematological markers. MATERIALS AND METHOD: The antioxidant and anti-hemolytic activity of Thuja orientalis was evaluated using the PAB assay and the inhibition of RBC hemolysis using the hydrogen peroxide hemolysis test. The percentage of anti-hemolysis was calculated from the ratio of the measurements (A-B)/B×100. FINDING: Our results showed that the antioxidant effect of Thuja orientalis L. was greater in water than in ethyl-acetate, ethanol and methanol extract, respectively. We also observed its anti-hemolytic effect, which was higher in water, than in ethyl-acetate, methanol and ethanol extract, respectively. In particular our data showed that the H2O2-induced RBC hemolysis was inhibited in a dose-dependent manner. CONCLUSION: We demonstrated the antioxidant and anti-hemolytic effect of Thuja orientalis L. extracts in human serum and RBC, showing its potential property of reducing free radicals supporting further investigations to assess its functional role in larger samples size and in vivo models, as a potential antioxidant agent.

Antioxidant; Diabetes mellitus (DM); PAB assay; Thuja orientalis L; anti-hemolytic; therapeutic approaches