M Mohammadinejad - Departmentof Materials Engineering, Faculty of Engineering, Islamic Azad University (Central Tehran Branch),Tehran,P.O. Box ۱۴۱۶۹-۳۳۱۱۶, Iran
H Farnoush - Department of Metallurgy and Materials Engineering, Faculty of Engineering, University of Kashan, Kashan, P.O.Box ۸۷۳۱۷-۵۱۱۶۷, Iran

Increasing coating adhesion on titanium surface among diverse methods is challenging. Over past two decades, enormous studies on metal implants showed that modifying the surface topography enables imitation of functional proteins and growth factors from host tissues and accelerates cell bond, proliferation, differentiation and, consequently, successful Osseo integration of the implants .The surface geography of titanium were modified by applying a combination of lithography and radio frequency magnetron sputtering (RFMS) techniques .The Micro-Nano structured hydroxyapatite-laponite composites were prepared in the form of disc shape with spark plasma sintering(SPS) method to form reliable dense sample as a target in RFMS method. SEM images verified high adhesion rate by applying RFMS method as a consequence of using sputtering force the material in angstrom scale make sediment in Nano scale hole. In addition The result of in-vivo study performed by Human mesenchymal stem cell not only has proved non -toxicity of coating, but also confirmed cell proliferation. The microstructure and phase of final coating were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), confocal laser-scanning microscope respectively

Magnetron Sputtering, Laponite,Hydroxyapatite, Spark plasma sintering, Human mesenchymal stem cell Adhesion