Genotoxicity and cytotoxicity of naringin nanosuspension prepared by microfluidic reactors on lela cell line

Introduction The present research applied nanoprecipitation process in microfluidic devices to prepare nanosuspension of stable naringin. Moreover, the present research aimed to investigate the induction of DNA destruction and colony formability of HeLa spheroid culture influenced by nanosuspension of naringin. ObjectivesThe nanosuspensions were used to improve cellular drug delivery and examine cytotoxic and genotoxic impacts of naringin nanoparticle encapsulations against the spheroid model of HepG2. MethodsThe culture of HeLa cells as spheroids was treated with different concentrations of naringin. Afterward, colony assay and alkaline comet assay methods, respectively, were applied to examine the viability and induced DNA destructions. ResultsBased on the results, no significant impacts of Tween 40 diluent were detected on and DNA damage levels in comparison with the control. Moreover, increasing naringin concentration resulted in a dose-dependent reduction in viability cells of HeLa cells and elevated DNA damages showing a correlation between rises of DNA damages and viability decline. Moreover, increasing naringin concentration resulted in a dose-dependent reduction in colony numbers of HeLa. ConclusionOur results show that nascapine is a good approach to be used as a choice for cancer treatment, furthermore as we demonstrate that, nanoparticle based nascapine delivery seems to be accurate, effective and safe method to treat the hepatic carcinoma cells line, beside that we clarify, nanoparticle synthesis procedure can make our encapsulated nascapine more applicable

Keywords:

Genotoxicity , Cytotoxicity , Naringin , Nanosuspension , Microfluidic reactors