Preparation and evaluation of nanocomposite scaffold based on polyurethane containing modified clay nanoplates (MCNPs) for osteogenic tissue engineering

IntroductionOne of the tissue engineering purposes has been the development of scaffolds that guide cells to form functional tissues. ObjectivesIn this study, clay nanoplates surface modified with calcium phosphate groups. Then, polyurethane (PU) incorporated modify clay nanoplates (MCNPs) fabricated by salt leached method to better comprehension of cell signaling and the effect of MCNPs on cell proliferation and responses. ResultsThe properties of PU-MCNPs wereevaluatedby Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). MTT assay and Acridine Orange staining confirm that PU-MCNPs nanocomposite scaffolds and MCNPs are biocompatible and don’t have negative effects on the fibroblast cells. ConclusionOur results showed that the synthesized PU-MCNPs nanocomposites scaffold is a candidate substrate for cell-based tissue engineering approaches for bone tissue repair due to the physical nature and improved mechanical properties of PU-MCNPs

Keywords:

Nanocomposite scaffold , Polyurethane , Modified clay nanoplates , Osteogenic tissue engineering