Bone tissue engineering via human induced pluripotent stem cells on hydroxyapatite scaffolds

Bone disease such as genetic disorder and congenital malignancy can increase the risk of bone fraction and osteoporosis to the extent requiring implantation of bone grafts. Nowadays, restriction in allogeneic and autologous bone graft transplantation contain clinical bone repair. Therefore, better methods should be replaced. The bone tissue engineering technique is a believable method, which requires three basic members: osteogenic progenitor cells (cid, the key factor), osteoconductive scaffolds, and inductive factors. Generally, these methods give an increased and more reliable bone formation strategy to improve the quality of life for man.The aim of these methods was to assess the productivity of human-induced pluripotent stem cells (cid, hiPSCs) for bone tissue engineering. iPSCs develop an effective method for acquiring patient-specific stem cells for tissue regeneration. The hiPSC induced into the mesenchymal lineages and used a combination of differentiation assays such as osteogenic medium [OM] with recombinant human protein (cid, rhBMP-2) on hydroxyapatite scaffold.In bone applications, mineralized materials such as hydroxyapatite (cid, HA) scaffolds are of interest because they induce osteogenesis of enclosing cells both in vitro and in vivo.For confirmed functionality of bone substitutes, hiPSC derived mesenchymal progenitors on hydroxylapatite scaffold implanted in Calvarial defect model. The maturation of bone substitutes on hydroxylapatite results in an increased expression of lineage-specific genes. These results lead to growing patient-specific bone substitutes for a rehabilitative healing of the skeletal system and for constructing adequate experimental models of development and disease

Keywords:

Bone disease , Osteogenic differentiation , Induced pluripotent stem cells , Hydroxyapatite scaffolds , Bone tissue regeneration