Effects of scaffold morphology on chondrocyte cells behavior for cartilage tissue engineering applicattion

IntroductionAs cartilage joint damages are extensively spread in the society and the ability to repair cartilage tissue in body is limited, achieving a suitable method for cartilage regeneration is an issue that has gained the attention of many scholars.ObjectivesFor this purpose, scaffold structure and morphology, along with cell culture on it, can be applied to pave the way for a novel method to treat cartilage injuries, osteoarthritis and cartilage repair.MethodsIn this study, polyhydroxybutyrate (PHB) is selected as the scaffold. Firstly, PHB (6% w/v) solution was prepared using chloroform solvent by employing two solvent and electrospinning methods. For this purpose, porosity of scaffolds was evaluated. In terms of phase studies, morphology, and specifying agent groups, specific characterization devices such as FT-IR were employed. To compare the behaviour of cellular scaffolds, they were divided into 2 groups of scaffolds, and the chondrocyte cells were cultured. To perform phase studies, analysis of MTT and Trypan blue were carried out for the viability and attachment on the surface of the scaffold, and the specification of SEM was employed for the morphology of the cells.ResultsIn solvent casting method, porosity percentage was obtained as 89.67, and in electrospinning method, it was obtained as 82.48. Through performing MTT test during one, three and seven days, it was found that these types of scaffolds are significantly different from the control group (p-value<0.05). Scanning electron microscope (SEM) indicates good attachment of chondrocytes on all scaffolds. Results obtained from Trypan blue exclusion test also indicated an increase in cell attachment on scaffolds.ConclusionScaffold morphology has effect on Cells Behavior

Keywords:

Scaffold morphology , Cells behavior , Cartilage regeneration