Design and fabrication of zinc-hydroxyapatite /agarose composite and evaluation of its antibacterial effect
Publish place: 1st International Iranian Tissue Engineering and Regenerative Medicine Congress (ITERM2018)
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ITERMED01_249
تاریخ نمایه سازی: 7 مرداد 1398
Abstract:
Composite powders are used for treatment of orthopedic diseases. Hydroxyapatite is the main mineral part of bone and teeth is used in bone and dental surgery. Agarose is a natural, thermally responsive polysaccharide that is widely used in biological applications (e.g., microbial cultivation and gel electrophoresis). Zinc is the most abundant trace metallic element found in bone and other hard tissues. Also this vital element effects on different biological functions while possess excellent antibacterial properties. Therefore, in this research Zn doped Hydroxyapatite/Agarose (HA/Agar) composite was synthesized as a good choice of biomaterial for orthopedic and dental applications and prevention of infection or inflammatory reaction after surgical implantation. The aim of this work was synthesizing Zn-HA/Agar micro powders containing various amounts of zinc (1and 2 wt % Zn) by sol-gel method and evaluating its antibacterial effect. X-ray diffraction (XRD), scanning electron microscopy (SEM), Atomic absorption spectroscopy (AAS) were used for characterization and evaluation of the phase composition, morphology and particle size of products. The size of microparticles was measured between 80-125 μm. The antibacterial activity was evaluated by using Escherichia coli gram negative, and Staphylococcus aureusas gram-positive bacteria. The antibacterial effect of the samples was investigated at concentration of 200 and 300 mg/ml. At concentrations of 200 and 300 mg/ml of the broth, the HA/Agar and HA/Agar with 1 wt% Zn had no antibacterial effect. However, HA/Agar-2wt% Zn particles at 300 mg/ml concentration after 48 hours had a significant antibacterial effect on both bacteria
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Authors
Shiva Ghanbarpour-Yasouri
Department of Biomedical Engineering, Maziar University , Noor, Iran
Zahra Nazemi
Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran
Masoumeh Haghbin-Nazarpak
New Technologies Research Center (NTRC), Amirkabir University of Technology, Tehran, Iran
Mehran Solati-Hashjin
Department of Biomedical Engineering, Amirkabir University, Tehran, Iran.