Histomorphometric assessment of alcoholic extract of grape seed (vitis-vinifera) on the renovation of diabetic wounds in male Wistar rats

IntroductionThe Process of wound healing happens with harmony of tissues, cells, and different factors. Wound healing is a function of regenerative response of blood cells, extracellular matrix and parenchymal cells that are carry out on the body after surgery or injury which is occurs in tissue rupture. The wound healing is divided into three phases of inflammation, proliferation and restructuring.Objectives1- To find an effective combination on the healing process of diabetic wounds2- To create a proper animal model3- Efficacy of black grape seed on histomorphometric changes of skin regeneration in diabetic male rats.MethodsForty-eight male wistar rats were divided into four groups: negative control, positive control, the first experimental and the second experimental. In all of treated rats, a scar in the length of three centimeters was created on the left of the vertebral column. The wound healing was evaluated microscopic as well as macroscopic.ResultsAgglutination in diabetic groups was longer than normal, while the process in the group treated with V. vinifera was quicker than the control and also first experimental groups, respectively. The methods Mann – Whitney, Kruskal – Wallis were used for data analyses. The used software was SPSS ver. 16. ConclusionComparing the results of this study with other results, it seems that the grape seed is probably due to the presence of anthocyanin with antioxidant effects, flavonoids with anti-inflammatory effects of Tannins and the effects of cell proliferation on the process of healing and repairing diabetic wounds by accelerating inflammation, Cell proliferation and hypoglycemia interfere with the repair of diabetic wounds.

Keywords:

Wound , Grape seed , Rat , Diabetes