Effects of size particles on mesenchyme stem cell behavior

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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ITERMED01_298

تاریخ نمایه سازی: 7 مرداد 1398

Abstract:

IntroductionCalcium phosphates have shown promising materials in bone tissue engineering (BTE) and size particles have modified the mechanical and biological characteristics of bone composite structures. Composite of polymers and ceramics, such as poly l-lactic acid (PLLA)/β-TCP (tri calcium phosphate) have been well evaluated for their proliferative and osteoinductive capabilities. Few researches have been investigated the effects of various sized β-TCP particles on stem cell behavior. Bodies are known to elicit an inflammatory and cytotoxic response and several nano-particles are known to elicit cytotoxic and pro-inflammatory responses of stem cells.ObjectivesIn this study, was investigated behavior of adipose-derived mesenchymal stem cell (hASC) exposed to particle size of β-TCP, in a polymeric-ceramic scaffold system.MethodsDifferent concentration of PLLA: β-TCP (90:10, 70:30, 40:60) structures using a modified unidirectional fabrication technique were constructed to investigate the cytotoxic, pro-inflammatory and osteogenic potential of PLLA-β-TCP composite on adult human stem cells.ResultsPLLA-β-TCP composites showed that increasing β-TCP content, the compressive strength decreases. Viability of hASCs over a 7 day exposure study showed increasing cytotoxicity with respect to increasing particle concentration regardless of size, although acute (24hr) cytotoxicity was more influenced by size. qPCR was used to measure the pro-inflammatory (IL-6) expression as well as early osteogenic differentiation (hALP). Results suggest there is a modulation in stem cell behavior as a function of β-TCP particle size, concentration and composite system.ConclusionNano sized particle was significantly stronger than their micro sized counterparts at each composition. Micro composites tend to be more bioactivity, but their reduced mechanical strengths make the unsuitable BTE

Authors

Mona Latifi

Institute of Genetic Engineering and Biotechnology, Tehran, Iran

Hossein Mehraban

Shiraz University of Medical Science, Shiraz, Iran.