Regenerative medicine in bilirubin encephalopathy (kernicterus)

IntroductionKernicterus is a neurological syndrome related with indirect bilirubin gathering and damages to the basal ganglia, cerebellum and brain stem nuclei mainly the cochlear nucleus.ObjectivesWe have studied the efficacy of human adiposity derived stem cells as a therapeutic pattern for perinatal neuronal restoration in a kernicterus animal model.MethodsTo copycat haemolysis in a rat model such that it was like to what is detected in a preterm human, we injected phenylhydrazine in 7-day-old rats to make haemolysis and then pervaded sulfisoxazole into the same rats at day 9.ResultsThe level of total bilirubin, indirect bilirubin, brain bilirubin and brain iron were significantly increased in the modelling group. There was a significant increasein inactivity in all severity levels of the auditory brainstem response test in themodelling group. Akinesia, bradykinesia and slip were significantly dropped in the experience group. Apoptosis in basal ganglia and cerebellum were significantly reduced in the stem cell cured group in comparison to the vehicle group. All severity levels of the auditory brainstem response tests were significantly diminished in 2-month-old rats. Transplantation marks the considerable mitigation of walking impairment, apoptosis and auditory dysfunction.ConclusionThis study offers important evidence for the progress of therapeutic strategies using human adiposity derived stem cells in prenatal brain injury to diminish potential sensorimotor deficit

Keywords:

Regenerative medicine , Kernicterus , Bilirubin Encephalopathy