Investigating the expression of FGF19 gene as an effective gene for the invasive behavior of cancer cells in patients with colorectal cancer

Introduction: Colorectal cancer is the second and third most common cancer in women and men respectively. Early diagnosis of illness will reduce pain and costs for patients. In this study, the expression of fgf19 gene in different individuals with colorectal cancer, and also the relationship between the expression of this gene and the degree and stage of cancer cells, will be evaluated. Methods: A total of 60 samples were collected by a surgeon from cancerous tissue and healthy marginal tissue of patients with colorectal cancer. Extraction of RNA was accomplished by a Trizol solution. In the next step, cDNA molecule was synthesized using reverse transcriptase enzyme (RT), and gene-specific primers were designed and synthesized. Then the expression of FGF19 genes were evaluated by Real-time PCR technique. Finally, the data obtained from cancerous tissue and healthy marginal tissue were analyzed by spss software. Result: The expression of fgf19 in tumor tissue and healthy marginal tissue was measured quantitatively, which increased by 56.7%.Conclusion: The results of this study indicate that fgf19 as a molecular biomarker plays a significant role in the progression of cancer, and therefore can be considered as a factor in the screening, early detection, prognosis and predictor of the tumor.

Keywords:

Colorectal Cancer , FGF19 , Tumor Tissue , RT-q PCR , Tumor Marker.