The Study of Rosa canina L Extract on Bcl_2 and Bax Genes Expression in Cancerous Cell Line AGS.

Introduction: Gastric cancer is the most common cancer in the world, with extensive research in the treatment of this disease. The BAX protein is a functional antagonist and is a structural homologous BCL-2 which can be accelerated to apoptotic BAX complex to death signaling, but in response to BCL-2 elevation and its heterodiagnosis with BAX, cell death is suppressed. Therefore, the expression ratio of BAX to BCL-2 determines the occurrence or absence of apoptosis. Rosa canina L is one of these herbal medicines. In this research we have studied the effect of Rosa canina L extract on Bax/Bcl2 gene expression ratio in adenocarcinoma gastric cancer cells. Methods: The AGS cells were incubated 37°C containing 5% CO2 with 85% humidity, DMEM with 10% FBS. The cells were treated with concentrations of 800, 1200, 2000 μg /ml of Rosa canina L extract for 48-72 hours. RNA extractin and synthesis of cDNA have been done using special kits. The study of BCL-2/BAX genes expression was performed by Real time PCR . Results: Analysis of results have shown that in all studied concentrations of Rosa canina L extract, there are significant increase in Bax/Bcl2 expression in AGS cell line. Increase in Bax/Bcl2 ratio in cells leads them to apoptosis. Conclusion: Rosa Calina changes the amount of Bax / BCL-2, which can transfer cancer cells to a planned cell death, so it can be useful in the treatment of gastric cancer.

Keywords:

BCL-2/BAX , AGS cells , Rosa canina L