The effects of hydrocortisone on tight junction genes in an in vitro model of the human fallopian epithelial cells

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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RMED08_082

تاریخ نمایه سازی: 21 مرداد 1398

Abstract:

Background: The tight junction between epithelial cells helps making connections in the fallopian tube and contributes to successful fertilization. Breaking the tight junction complex induces various diseases such as the EP. Previous studies have shown that glucocorticoids are effective in repairing and maintaining intercellular tight junctions in epithelial cells of the fallopian tube, although their mechanism is still unknown.Objective: This research is a genomic study of hydrocortisone’s effect on epithelial cells of the fallopian tube.Materials and Methods: Using the human fallopian tube, epithelial cell line (OE-E6/E7) was cultured in four concentrations of hydrocortisone (0 nM, 50 nM, 100 nM and 200 nM) for three durations (24, 48 and 72 hr).Results: Glucocorticoids are effective on the expression of Zona occluding-1 (ZO-1), Claudin 4, Claudin3, Desmoglein and E-cadherin genes involved in the tight junctions of the fallopian tube. The expression of all genes was up-regulated in the concentrations of 100nM after 48h treatment, as compared with the control (0nM). However, their expression was down-regulated significantly after 72h treatment (p<0.05).Conclusion: The present study showed that treatment of epithelial cells of the fallopian tube with glucocorticoid increased the expression of genes involved in tight junctions, including claudin-3, claudin-4, E-cadherin, zona occludin-1 and Desmoglein-1. The obtained data suggests that a new mechanism is developed for glucocorticoid induction of tight junctions by increasing the expression of claudin-3, claudin-4, E-cadherin, zona occludin-1 and Desmoglein-1 genes.

Authors

S Zhaeentan

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

FS Amjadi

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

M Bakhtiyari

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

M Joghataei

Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran