Is there any relationship between human sperm parameters and protamine deficiency in different groups of infertile men

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
View: 376

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

  • Certificate
  • من نویسنده این مقاله هستم

این Paper در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

RMED08_104

تاریخ نمایه سازی: 21 مرداد 1398

Abstract:

Background: Abnormality in Histone- Protamine replacements have been indicated to cause sperm DNA damage and infertility.Objective: The aim of present study was to investigate the relationships between sperm parameters in oligospermia, asthenospermia, and teratospermia with protamine deficiency in infertile men.Materials and Methods: In this case-control study, we had three experimental groups including oligospermia (n=100), asthenospermia (n=100), and teratospermia (n=100) and also normospermia (n=100) as controls. Sperm analyses were done according to the recommendations of the World Health Organization (WHO, 2010) and sperm chromatin quality was assessed using Chromomycin A3 (CMA3) staining for each sample.Results: Sperm concentration and normal morphology were significantly different between all experimental groups as well as controls (p<0.05), except those between oligospermia and asthenospermia semen samples. The comparison of the data between groups indicated that the percentage of spermatozoa with protamine deficiency was significantly different in patients with oligospermia, asthenospermia, and teratospermia when compared with control ones. However, There was no significant difference between sperm nuclear protamine deficiency of the men with teratospermia and oligospermia using CMA3 test (p=0.172).Conclusion: The higher proportion of spermatozoa with abnormal chromatin packaging was observed in asthenospermic samples than those from other experimental as well as control. The data indicate that chromatin quality assessments could apply as the key confirmatory indicator beside the semen analysis in infertile men, as well.

Authors

F Dehghanpour

Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Fesahat

Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Yazdinejad

Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

L Motamedzadeh

Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran