Sperm HSP90 transcript content in normo- and asthenozoospermic men

Background: There is a complex of mRNA transcripts in ejaculated sperm that participate in many cellular processes.Objective: Present study was done to evaluate mRNA content of HSP90 in ejaculated sperm of normozoospermic and asthnozoospermic men.Materials and Methods: Present study was approved by the institutional review board of Biology Department, Shahid Chamran University, Ahvaz, Iran. Sperm parameters were analyzed according to the World Health Organization (WHO) guidelines in men attending an infertility clinic and categorized to normozoospermic and asthnozoospermic groups. Real-time PCR was carried out to evaluate mRNA content of HSP90 in ejaculated sperm.Results: Sperm content of HSP90 transcript was significantly (p<0.05) higher in asthnozoospermic than normozospermic men. Significant (r=-578, p<0.01) correlation was seen between sperm mRNA content of HSP90 and sperm motility.Conclusion: These findings show that sperm mRNA content of HSP90 has important clinical significance and could be considered as a diagnostic tool in prediction of male infertility.

Keywords:

Semen quality , Asthenozoospermia , HSP90 , Transcriptome.