Study of side effects of biolozax-H on testis tissue and epididymal sperm of adult rat following treatment with clinical dose

Background: The activity of the renin-angiotensin system has an effect on oxidative stress in many tissues, including the testes. Therefore, in this study, the biolozax-H drug, which has an effect on the spermatogenesis activity and sperm quality in male rats, is further investigated.Objective: The aim of this study was to assess side effects biolozax-H dose of biolozax-H on testis and epididymal sperm of adult male rat.Materials and Methods: Male adult rats (200-250 gr) were divided into two groups of sixteen each. Control and biolozax-H treated group were administered 20 mg/kg biolozax-H for 45 days, respectively. The animals were sacrificed 45 days after starting treatment. The histological change on germinal epithelium, sperm quality parameters (count and normal morphology), viability and DNA fragmentation on sperm were analyzed by light microscopy, CASA (Computer-aided Sperm Analyzer).Results: Biolozax-H administration significantly decreased parameters of sperm (count and normal morphology) and increased germinal epithelium destruction. The head, mid piece and tail abnormalities of treated group were increased significantly versus control. Higher levels of TUNEL positive cells that were found in treated groups demonstrated the increasing of DNA fragmentation in sperms following biolozax-H treatment.Conclusion: The results showed that clinical dose of biolozax-H induces genotoxic, pre-apoptotic and cytotoxic effects on spermatogenesis. Also, biolozax-H can increase cytotoxicity on testis tissue of adult male rat.

Keywords:

biolozax-H , Epididymal sperm , Sperm viability , Testis