Effect of GDF9 and CCs supplementation in IVM culture media on viability of blastocyst generated from vitrified clavage embryos

Background: In vitro maturation (IVM) of immature oocytes is useful for infertile patients such as Polycystic Ovary Syndrome (PCOS). Embryo formation and Pregnancy rates in IVM oocytes are low in comparision with IVF cycles.it is essential to recognize the main factors in regulation the oocyte maturation. This can be referred to the cobe culture system and GDF-9 which is released by the oocyte.Objective: The purpose was to investigate the effects of GDF-9 and cumulus cells (CCs) supplementation M I ntlucure media on the formation and viability rates of blastocysts generated from c ca s ig tuircrti fcrtiioyabmf.Materials and Methods: A total of 80 GV oocytes from stimulated cycles underwent the IVM program. These oocytes were divided into four groups of I) cultured in IVM media, II) IVM+ CCS, III) IVM+GDF-9 (200 ng/ml) and IV) IVM medium+ CCS+ GDF-9 (200 ng/ml). Intracytoplasmic sperm injection (ICSI) was performed for the IVM oocytes and the generated cleavage embryos were vitrified for future analysis. Following thawing, the embryos were cultured for 3 days and the developed blastocysts were assessed for viability rates with PI/ hochest immunostaining.Results: Blastocyst formation was not significant in the three treatment groups compared to the control (group I). The mean of viability rates in the II, III and IV groups were 57.82%, 57.75% and 59.9% respectively, versus 38.78% in the control group. The rates of blastocyst viability in II, III and IV groups were significantly higher than the control group.Conclusion: The use of GDF-9 CCs in IVM culture media enhanced the viability rate of the generated yurfcmtbfcf samo c ca s ig tuircrti ioyabmfa bherefore, modification of IVM culture media is aitmooiMgig.

Keywords:

IVM , GDF-9 , Cumulus cells , Blastocyst formation