CIVILICA We Respect the Science
(ناشر تخصصی کنفرانسهای کشور / شماره مجوز انتشارات از وزارت فرهنگ و ارشاد اسلامی: ۸۹۷۱)

An investigation of the effect of the human cumulus cell conditioned medium on mouse oocytes in vitro maturation

عنوان مقاله: An investigation of the effect of the human cumulus cell conditioned medium on mouse oocytes in vitro maturation
شناسه ملی مقاله: RMED08_240
منتشر شده در هشتمین کنگره بین المللی و جشنواره دانشجویی طب تولید مثل و سومین کنگره بین المللی ژنتیک تولید مثل در سال 1398
مشخصات نویسندگان مقاله:

R Rezaee-Ranjbar-Sardari - Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran- Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Research and Clinic
M Adib - Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran- Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Research and Clinic
A Khoradmehr - Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
F Mazaheri - Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

خلاصه مقاله:
Background: Since defect of oogenesis is the cause of infertility in some infertile couples, in vitro maturation (IVM) of germinal vesicle (GV) oocytes is an important option in infertility treatments. Cumulus cells are essential for nutrition of oocytes during oogenesis. They provide a suitable environment contains growth factors required for oogenesis. It was shown that cumulus cell conditioned medium (CCCM) supports female germ cell development from Buffalo embryonic stem cells (ESCs).Objective: In this study the impact of CCCM on murine IVM was investigated.Materials and methods: NMRI female mice, aged 4-6 weeks, were injected with 5 IU of Pregnant Mare’s Serum Gonadotropin (PMSG) intrapritoneally (IP). 240 germinal vesicle (GV) oocytes were obtained. GV oocytes without cumulus cells were cultured in CCCM (test group; 120 GVs) and DMEM+10%FBS (control group; 120 GVs). GV maturation rate was checked by microscopic observation at 8, 16 and 24 hours following IVM in both groups.Results: Significant difference was observed between CCCM (19.16%) and control (0%; p<0.05) following IVM.Conclusion: Human CCCM supports IVM in mice oocytes. For further investigations we aim to evaluate the effect of TCCM on human IVM and assess the metabolomics profile of TCCM in our future studies.

کلمات کلیدی:
Conditioned Medium, Cumulus Cells, Germinal Vesicle, In Vitro Maturation

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/912333/