Evaluating expression level of the circulating mir-146 in under treatment epileptic patients as a noninvasive biomarker of PCOs

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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RMED08_270

تاریخ نمایه سازی: 21 مرداد 1398

Abstract:

Background: Epilepsy is a common chronic neurological disorder affecting approximately 50,000,000 people by recurrent unprovoked seizures. However, there is increasing concern that the pathogenic epilepsy mechanism remains poorly defined. Antiepileptic drugs are known to have endocrine side effects. Valproic acid (VPA) is widespread drug in the treatment of epilepsy. In women, VPA can also lead to androgenization, menstrual disturbances, and polycystic ovaries. The incidence of polycystic ovary syndrome (PCO) increases in women with epilepsy (WWE), which appears to vary with ethnicity.Objective: Main aim of this study is determining a non-invasive biomarker in order early diagnosis of PCOs in WWE patients for preventative measures.Materials and Methods: The study was carried out in 15 WWE women with PCO at reproductive ages (18-40 yr), monotherapy with VPA and 15 healthy women as control. Excluded criteria were history of diabetes, kidney and liver disease. After collecting patient’ serum, total RNA was extracted and Q-RTPCR was used to identify the expressions level of miRNA-146.Results: In accordance with previous studies on PCOs and non-PCOs women, expressions level of miRNA-146 were significantly different between epileptic patients with PCO and without it (p<0.05).Conclusion: Our results suggesting miRNA-146 as non-invasive biomarker to screen for drug side effects and prevent the progression of its complications. Furthermore, bioinformatics analysis indicate role of miRNA-146 in insulin signaling pathway and increasing estradiol secretion which may be of importance in PCOs.

Authors

M Rajabi

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Montazeri

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

S Hosseini

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

M Etemadifar

Department of Neurology, Isfahan University of Medical Sciences, Isfahan, Iran