Downregulation of MICAL3 in breast cancer

Introduction and Aim: Breast cancer is the most commonly diagnosed cancer in women. Female breast cancer is a major medical problem with significant public health and societal ramifications. Despite major advances that have been made in the past years and notwithstanding dramatic changes in its treatment, the problem continues to persist and has become more complex. So, determining molecular changes for diagnosis and prognosis of this cancer is important. MICAL3 plays important regulatory functions in actin cytoskeleton organization, apoptosis, membrane trafficking and etc. In this study, the expression of MICAL3 was analyzed. Methods: Total RNA was extracted from sixty pairs of specimens, breast tumor and their marginal tissues, using RNX-plus reagent and cDNA was synthesized by PrimeScriptTM RT reagent kit(Takara). Expression of MICAL3 was evaluated by qRT-PCR. GAPDH was used as internal control gene. Results: The relative expression of MICAL3 showed significantly downregulation in tumoral tissues compared to the paired non-tumoral samples (fold change: 0.410, p-value: 0.03). Moreover, our findings showed that there was a significant correlation between metastasis and expression level of MICAL3. Conclusion: apparently MICAL3 is a factor involved in breast cancer. Cytoskeleton regeneration is a vital event in the progression of metastasis, since it regulates the main characteristics of the cell such as proliferation, differentiation, cell-cell adhesion, or cell-mobilization, mobility and invasion, all of which contribute to the onset and progression of cancer.

Keywords:

breast cancer , MICAL3 , qRT-PCR , Cytoskeleton