Investigation on the Effect of Quercetin Suspended Nanoparticles on Cancer stem cell Breast Cancer

Introduction & Aim: Breast cancer is one of the most prevalent mortality factors among the women all over the world. Breast cancerous tumors are derived from breast carcinogenic cells, but few of them are able to generate new tumor. In fact, these cells are called cancerous stem cells, which are diagnosed by the superficial symptoms such as CD44+/CD24. Quercetin is a bioactive compound with plant origin, which has antioxidant and free radical scavenging properties as one of the most considerable properties enabling quercetin to inhibit composed of cells growth and activate apoptosis pathways with different composed of cells mechanisms. In this study, Quercetin has been converted into Quercetin na no-suspension through stages using microfluidic tools and hydrodynamic pump. Na no-suspensions have good solubility and don’t cause toxicity in natural cells. Aim of this study is to study an effect of Quercetin na no-suspension toxicity on breast cancer stem cells. Materials and method: MDA-MB 231, MCF7cells were exposed as the experimental groups to concentration of 40 μM/ml of Quercetin and Quercetin na no-suspension. Effect of Quercetin and Quercetin na no-suspension on growth of MCF7, MDA-MB 231 was assessed with MTT technique, scratching test, colony test, and spheroid test. Results: MTT technique didn’t show a significant effect, but it was shown in another technique that Quercetin na no-suspension can create considerable toxic effects on MCF7, MDA-MB 231 cells as compared to ordinary quercetin. Conclusion: these results show that use of Quercetin na no-suspension causes to increase anticancer property.