Effect of Hydroxyapatite Nanoparticles on Spin-Spin Relaxation Times of Aminoacids in Early Stages of Gelatin Gelation

This research focused on the gelation process of gelatin after mineralization with nano-particulate hydroxyapatite and investigation of relaxation behavior of aminoacids in gelatin chains and changes in the coil structure of 3 and 5% gelatin solution under variation of temperature (273-330 K). Spin-spin relaxation time variations are related to the large scale molecular mobility of molecules in solvent. The NMR state diagrams, i.e. plots of ln (T2) vs. the reciprocal temperature (1/T), were produced for 3 % and 5% gelatin solutions mineralized with hydroxyapatite. We found that the different concentrations (3 and 5%) have no drastic effect on the T2 relaxation time but different aminoacids have different patterns, so a different mobility during heat scan can be postulated. It may relate to this fact that they are well designed to have an interaction with phosphate and calcium anion.

Keywords:

gelatine , hydroxyapatite , spin-spin relaxation , aminoacids