In vitro capacity and in vivo antioxidant potency of sedimental extract of Tinospora cordifolia in streptozotocin induced type 2 diabetes

Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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JR_AJP-3-1_002

تاریخ نمایه سازی: 1 مهر 1398

Abstract:

Objective: The role of herbs against the free radicals have been put forth recently in combating many diseases. The aim of this study was to elucidate the in vitro capacity and in vivo antioxidant properties of sedimental extract of Tinospora cordifolia (SETc). Materials and Methods: SETcwas subjected to in vitro chemical analysis such as 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, hydrogen peroxide, and superoxide anion radicals scavenging respectively and finally drugs reductive ability in order to elucidate the antioxidant capacity of the test drug before introducing it into the biological membrane. The resulting capacity was evaluated in vivo by analyzing enzymic (SOD, CAT) and non-enzymic (vitamin C & E) antioxidant levels in the homogenized samples of major organs isolated from streptozotocin induced type 2 diabetic rats after 30th day of SETc (1000 mg/kg/p.o.) treatment. Finally, the histopathological evaluation was done using cut portion of the respective organs prone to free radical mediated cell destruction with STZ in order to study their micro anatomical changes. Results: Chemical analysis with SETc in vitro for its IC50 proves a key evident for its total antioxidant capacity of around 2046 times, in 1000 mg/kg of fixed dose per oral for in vivo analysis. In contrast to the above, the lipid peroxide levels and in vivo enzymic and non-enzymic antioxidant levels were found to possess most significant difference (p<0.001) and moderate difference (p<0.01) with diabetic non-treated animals which was an supporting contribution for those in vitro parameters studied and have proved that SETc (1000 mg/kg/p.o.) was a potent drug to elevate the antioxidants levels and further healing of damaged organs as compared with that of diabetic and standard drug treated groups. Conclusions: Finally, it was concluded that, the presence of antioxidant potentials in SETc was about 2046 time as an effective scavenger of free radicals in vitro and as a potent healer in ameliorating many signs of tissue damages in vivo in long term complicated diseases such as diabetes.

Keywords:

Antioxidants , Sedimental Extract of Tinospora Cordifolia (SETc) , Streptozotocin (STZ) , Type 2 diabetes

Authors

Ramachandran Kannadhasan

Research Associate, Dr.C.L.Baid Metha Foundation for Education and Research, Thoraipakkam, Chennai-۶۰۰ ۰۹۷, Tamil nadu, India

Subramaniam Venkataraman

Research Associate, Dr.C.L.Baid Metha Foundation for Education and Research, Thoraipakkam, Chennai-۶۰۰ ۰۹۷, Tamil nadu, India

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  • Arulmozhi S, Mazumder PM, Purnima A, Sathiya Narayanan L. 2007. ...
  • Atanaisu RL, Stea D, Mateescu MA, Vergely C, Dalloz F, ...
  • Aurand LW, Boone NH, Giddings GG. 1977. Superoxide and singlet ...
  • Blois MS. 2001. Antioxidant activity of grape seed extracts on ...
  • Baynes JW. 1991. Role of oxidative stress in the development ...
  • Chattejee IB, Nandi A. 1991. Ascorbic acid: A scavenger of ...
  • Chopra K, Singh M. 1994. Involvement of oxygen free radicals ...
  • Cotelle A, Bernier JL, Catteau JP, Pommery J, Wallet JC, ...
  • Desai ID. 1984. Vitamin E analysis methods for animal tissues. ...
  • Dhanukar SA, Kulkarni RA, Rege NN. 2000. Pharmacology of medicinal ...
  • Erika Szaleczky, Jozsef Prechl, Janos Feher, Aniko Somogyi. 1999. Alterations ...
  • Halliwell B, Gutteridge JM, Auroma OI. 1987. The deoxyribose method: ...
  • Harborne JB. 1984. Phytochemical methods: A guide to modern techniques ...
  • Hideaki K, Yoshitaka K, Jun-ichiro M. 1999. Beneficial effects of ...
  • Jay Prakash GK, Singh RP, Sakariah KK. 2000. Antioxidant activity ...
  • Kanai Mukherjee. 1989. Medical Laboratory Technology, Ist Edn, p. 124, ...
  • Kannadhasan R, Venkataraman S. 2011. Blood Glucose Concentration - A ...
  • Liu F, Ng TB. 2000. Anti oxidative and free radical ...
  • Marklund S, Marklund G. 1974. Involvement of the superoxide anion ...
  • Matkovica B. 1977. Effect of plant and animal tissue lesions ...
  • Matkovica B, Varga SI, Szabo L, Witas H. 1982. The ...
  • Nagaraja Puranik K, Kammar KF, Devi S. 2007.  Modulation of ...
  • Okutan H, Ozcelik N, Yilmaz HR, Uz E. 2005. Effects ...
  • Omaye ST, Turnbull TD, Sauberlich HC. 1979. Selected method for ...
  • Ozkaya YG, Agar A, Yargicoglu P et al. 2002. The ...
  • Patel MB, Mishra S. 2011. Hypoglycemic activity of alkaloidal fraction ...
  • Prince PS, Kamalakkannan N, Menon VP. 2004. Restoration of antioxidants ...
  • Rajalakshmi M, Eliza J, Cecilia Edel Priya, Nirmala A, Daisy ...
  • Rao MV, Rao EV. 1981. Studies on the polysaccharide preparation ...
  • Robert D. Hoeldtke, Kimberly D. Bryner, Daniel R. Mc Neill. ...
  • Sainani GS, Manika JS, Sainani RG. 1997. Oxidative stress- a ...
  • Sangeetha MK, Balaji Raghavendran HR, Gayathri V, Vasanthi HR. 2011. ...
  • Senthil Kumar GP, Subramaniam S. 2007. Evaluation of antioxidant potential ...
  • Shivananjappa MM, Muralidhara. 2012. Abrogation of maternal and fetal oxidative ...
  • Sinha AK. 1972. Colorimetric assay of catalase. Anal. Biochem, 47: ...
  • Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, ...
  • Soon YY, Tan BKH. 2002. Evaluation of Hypoglycemic and Antioxidant ...
  • Sreejayan N, Rao MNA. 1997. Nitric oxide scavenging by curcuminoids. ...
  • Sreenivasa Reddy S, Ramatholisamma P, Ramesh B, Baskar R, Saralakumari ...
  • Stanely Mainzen Prince, P, Padmanabhan M, Venugopal Menon P. 2004. ...
  • Sugiura M, Ohshima M, Ogawa K, Yano M. 2006. Chronic ...
  • Suryanarayana P, Satyanarayana A, Balakrishna N, Uday Kumar P, Bhanuprakash ...
  • Takenaka Y, Miki M, Yasuda H, Mino M. 1991. The ...
  • Uchiyma M, Mihara M. 1978. Determination of Malondialdehyde precursor in ...
  • Wohaieb SA, Godin DV. 1987. Starvation-related alterations in free radical ...
  • Yagi K. 1976. Simple fluorimetric assay of lipid peroxide in ...
  • Yilmaz HR, Uz E, Yucel N et al. 2004. Protective ...
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