Ethylene Glycol Induced Calcium Oxalate Crystals and Oxidative Damage of Renal cells

Introduction: : Urolithiasis is one of common renal disease that attributed to multiple factors. In otherhand, calcium oxalate is the most common urine lithiasis and urinary calcium and oxalate oversaturationare both important to calcium oxalate stone formation. The percent study was undertaken to examine thepossible role of oxidative stress and cell injury in stone formation.Method: In this research 12 rats with average weight of (200-250) ±6 grams in two groups, accidentallywere chosen. Stone group animals consume ethylene glycol 0.75% and ammonium chloride 1% for threedays and then ethylene glycol 0.75% for 25 days to create stones in their kidneys. Control group ratsmaintained on regular food and drinking for 28 days. The status of oxidative stress induced by Calciumoxalate was evaluated by malondialdehyde and superoxide dismutase in renal tissue.Findings: In present study, the body weight gain was small in stone group compare control group butkidney weight increased significantly. Many crystals deposit of Caox were seen in all regions of renaltubules in stone group. Calcium excretion increased after stone formation but magnesium decreasedsignificantly. Urinary creatinine content was higher in control group. The serum creatinine, urea and uricacid were remarkably increased in urolithiasis induced group compare with control group. Superoxidedismutase activity decreased significantly in stone groups but MDA production increased in stone groupwhen compare with control group.

Keywords:

Urolithiasis , Calcium Oxalate , Malondialdehyde , Superoxide Dismutase