Mohammad Mousaei Ghasroldasht - Department of Biology, Ashrafi Esfahani University, Esfahan, Iran Department of Orthopedic Surgery, AJA University of Medical Sciences, Tehran, Iran
Amir Moayednia - Shahid Sadooghi Hospital, Isfahan, Iran
Mostafa Shahrezaee - Department of Orthopedic Surgery, AJA University of Medical Sciences, Tehran, Iran

Background: Intra-articular injection of Platelet-rich plasma (PRP) is an effective method for the treatment of patientswith knee osteoarthritis (OA). This study aimed to assess the effects of PRP injection on OA, based on gene expressionanalysis.Methods: A sample of 30 subjects with knee OA was asked to complete the Persian versions of the Intermittent andConstant Osteoarthritis Pain (ICOAP) and Knee and Osteoarthritis Outcome Score (KOOS). Thereafter, the expressionof IGF-1, HIF-1, cartilage oligometric matrix protein (COMP), and bone morphogenetic proteins (BMP2) were comparedin the patient before and 1 month after PRP injection using real-time polymerase chain reaction (PCR).Results: According to the results of the study, the expression of IGF-1, HIF-1, COMP and BMP2 were reported to behigher in subjects with PRP injection; however, only the up-regulation of IGF-1 was statistically significant (P<007).Moreover, the significant change in the KOOS and ICOAP scores was attributed to PRP injection (P<0.01).Conclusion: Intra-articular injections of PRP were reported to ease the pain, decrease the stiffness, and improvequality of life in patients with knee OA through the promotion of IGF-1 expression.Level of evidence: V

IGF-1, Osteoarthritis, Platelet-rich plasma