Emmanuel Khan Muluh - Department of Chemistry, College of Science, university of Agriculture, Makurdi Nigeria
Agustine Odokpe Ugbede - Department of Chemistry, College of Science, university of Agriculture, Makurdi Nigeria
Terumon Amon Tor-Anyin - Department of Chemistry, College of Science, university of Agriculture, Makurdi Nigeria

Study evaluates the in-vitro anti-microbial and anti-ulcer activity of Cassia sieberiana leaf extracts on Helicobacter pylori the major etiological agent of chronic active gastritis and peptic ulcer disease also called gastric carcinoma. Crude extracts of cassia sieberiana were screened for their antimicrobial and antiulcer activities. Antimicrobial assay and in-vitro anti-ulcer (H. pylori) was performed using Agar Diffusion Method and Broth Dilution Method. Ethyl acetate extract had the highest diameter of zones of inhibition (30 mm). Ethyl acetate and methanol extracts had the same Minimum inhibitory concentrations of 10 mg/mL against Streptococus feacalis, Escherichia coli, Helicobacter pylori, shigella dysenteriae and Candida stellafoidea but for Campylobacter jejuni which had a minimum inhibitory concentration at 20 mg/mL for methanol extract. Methanol extract had the best minimum bactericidal/fungicidal concentration (40 mg/mL) on the pathogens. The results showed that Cassia sieberiana exhibited strong antimicrobial activity against H. pylori at two different concentrations of 20, 40mg/mL.

in-vitro, anti-microbial, anti-ulcer activities and pathogens inhibition