An Association Analysis of Reelin Gene (RELN) Exon 22 (G/C), Rs.362691, Polymorphism with Autism Spectrum Disorder among Iranian-Azeri Population

Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_INJPM-4-7_001

تاریخ نمایه سازی: 20 مهر 1398

Abstract:

Background Autism spectrum disorder (ASD) is a intricate childhood neuropsychiatric disorder that is described by deficits in communication of verbal and non-verbal, reciprocal social interactions, stereotypic behaviors, interests, and activities. The studies of post-mortem neuro-anatomical anomalies have indicated that migration alterations could occur early during development (first trimester) in autistic brain. Since the Reelin gene, plays a crucial role in these migratory processes, it is subsequently considered as a potential candidate gene for autism. Materials and Methods In this case-control study, we recruited 74 patients with ASD and 88 healthy controls from Iranian-Azeri Population. Genomic DNA isolated from blood leukocytes of cases and control individuals by the proteinase K and using salt-out method. Single nucleotide polymorphisms (SNP) genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results The allele and genotype frequencies did not show significant difference between autistic and control groups (P> 0.05). No significant relationship was observed between the genders and genotypes in autism group (P> 0.05). Conclusion The current study showed that the SNPs rs362691 could not be used as a useful molecular biomarker to predict genetic susceptibility for ASD among Iranian-Azeri patients.

Authors

Leila Mehdizadeh Fanid

Assistant Professor of Cognitive Neuroscience. Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, ۲۹ Bahman Bolvard, Tabriz, Iran, IR.

Mohammad Ali Hosseinpour Feizi

Professor of Radiobiology, Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, ۲۹ Bahman Bolvard, Tabriz, Iran, IR.

Mina Adampour Zare

Physiologist, Department of Animal Biology Faculty of Natural Sciences, University of Tabriz, ۲۹ Bahman Bolvard, Tabriz, Iran, IR.

Hasan Shahrokhi

Child and Adolescent Psychiatrist MD, Research Centre of Psychiatry and Behavioral Science, Tabriz University of Medical Science, Tabriz, Iran, IR.