Le Hien - Institute of Genome Research, Vietnam Academy of Science and Technology, ۱۸ Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam|Graduate University of Science and Technology, Vietnam Academy of Science and Technology, ۱۸ Hoang Quoc Viet, Cau Giay, Hanoi,
Phi Trang - Institute of Genome Research, Vietnam Academy of Science and Technology, ۱۸ Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
Pham Phuong - Nuclear Medicine and Oncology Center, Bach Mai Hospital, ۷۸ Giai Phong, Hanoi, Vietnam
Pham Tam - Hanoi Open University, ۱۰۱ Nguyen Hien, Hai Ba Trung, Hanoi, Vietnam

Objective(s): Copper (Cu) is an essential dietary supplement in animal feeds, which plays an important role in maintaining the balance of all living organisms. Copper nanoparticles (nCu) participate in catalysing activities of multiple antioxidant/defensive enzymes and exerts pro-inflammatory and pro-apoptotic effects on systemic organs and tissues. The present study explored whether nCu affects maize growth and yield and grain mineral nutrients as well as physiological functions in mice. Materials and Methods: Maize seeds were treated with nCu (20 mg/kg and 1000 mg/kg dry weight (DW)) and their grain productions were used for mouse feed. For testing of autoimmune response, mice were treated with nCu at concentration of 2 mg/l and 1000 mg/l and ultimately serum biochemical indicators, numbers and activation of immune cells infiltrated in mouse spleens were examined. Results: Treatment of maize seeds with nCu at dose of 20 mg/kg DW, but not 1000 mg/kg DW enhanced germination rate, plant growth and grain yield as well as grain mineral nutrients as compared to control group. Importantly, administration of mice with 1000 mg/l nCu resulted in their morphological change due to excessive accumulation of nCu in liver and blood, leading to inflammatory responses involved in upregulated expression of serum biochemical indicators of liver and kidney as well as increased infiltration and activation of splenic immune cells. Conclusion: nCu concentration at 20 mg/kg DW facilitated the morphological and functional development of maize plants, whose production was safe to feed mice.

ALT, AST, Copper, Leukocytes, Maize