Luteinizing hormone changes in gonadotropin-releasing hormone antagonist protocol in in vitro fertilization cycles: A cross-sectional study

Background: Because of the unexpected and often dramatic inhibition of luteinizinghormone (LH) secretion related with the usage of gonadotropin-releasing hormone(GnRH)-antagonist, there has been a probable need for exogenous LH supplementation.There is a basic and clinical evidences that show late development of follicleneeds an LH but there is a threshold for LH requirements during folliculogenesis.Objective: The purpose of this study was to evaluate the changes in serum LH and theidentification of patients who benefit from the addition of LH.Materials and Methods: Seventy volunteers for antagonist protocol in IVF cyclewere enrolled in this prospective cross-sectional study. The study was carried out inReproductive Health Research Center, University of Medical Sciences between July2016 and February 2016. Serum LH level was estimated 24 h before and after the first(GnRH) antagonist injection. The primary outcome was the serum level of LH and itschange in the three groups and the secondary outcome was Egg and Embryo quality.Results: LH changes above or below 50% had no effect on the number of follicle, thenumber of oocyte, Germinal vesicle oocyte, metaphase 1 oocyte, metaphase 2 oocyte,endometrial thickness, and chemical and clinical pregnancy.Conclusion: We evaluated the changes of serum LH in the patients who were enteredin the antagonist protocol. Our study showed no significant difference in LH levels 24h before and after the injection of the antagonist between the three groups, and LHchanges did not affect the outcome of pregnancy.

Keywords:

Gonadotropin-releasing hormone-antagonist , Luteinizing hormone , In vitro fertilization