Chondrogenic Related Genes Expression on IGF1-Induced Synovial Membrane Mesenchymal Stem Cells Iso-lated from Osteoarthritis Patients

Background: Osteoarthritis (OA) is the most common joint disease that affect more aged-people and decline the quality of life. Autologous chondrocytes implantation (ACI) is current popular cell-based treatment for OA patients. However, this therapy has several disadvantages due to the involvement of surgery procedure. Mesenchymal Stem Cells (MSCs) has capa-bility to differentiate become several cell types including adi-pocytes, chondrocytes and osteocytes. Enhancement of MSCs differentiation can be the alternative approach for cell-based therapy. IGF-1 is one of growth factor that regulate the chon-drogenic differentiation. This study is aimed to evaluate the dif-ferentiation potency of MSCs derived from synovial membrane of OA patients (SMMSCs) with various concentration of IGF-1 (0, 75, 150, 300 ng/mL) as inducerMaterials and Methods: The evaluation of SMMSCs differ-entiation was performed by chondrogenic markers expression such as SOX9, ACAN, and COL2 by real time PCR analysis. Results: The concentration 150 ng/mL of IGF-1 produced the highest level of all gene expressions among the group treat-ments both in a week and two weeks of treatment. It was shown a significant increase of the expression levels during two weeks of treatment. Aggrecan is shown to have the highest level of expression among the other markers which means the change in ECM matrix during chondrogenesis is already started from the second week.Conclusion: From this study, 150 ng/mL of IGF-1 is the best concentration for inducing chondrogenic differentiation.

Keywords:

Synovial Membrane Mesenchymal Stem Cells , Chondrogenesis , IGF-1 , SOX9 , COL2