Microglia Populations in The Brain Cortex, Hip-pocampus, and Thalamus: A Preliminary Study To Show the Relationship between Their Populations and Neurore-generation Activity

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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RROYAN20_034

تاریخ نمایه سازی: 29 مهر 1398

Abstract:

Background: Microglia release a range of regeneration factors in the injured nervous system. They are main cells of the im-mune system in central nervous system and play an essential role in nervous systems development and repair. Microglia rel-evant to most central nervous system diseases such as migraine, Alzheimer s disease, and Parkinson s disease, are related to cor-tex, hippocampus, and thalamus, respectively. The aim of our preliminary study was to evaluate the microglia’s populations and their microstructures in the cortex, hippocampus, and thala-mus of normal mice.Materials and Methods: Mice brain coronal slices were stained with ionizing calcium-binding adaptor molecule 1 (Iba1) antibody and imaged with confocal microscopy. Differ-ent parts of cortex, hippocampus, and thalamus of mice brain slice (five micrometer) were cropped and two dimensionals im-aged analyzed using Fiji and Imaris software programs. Various indices such as number of microglia in the 500 µm2 area, levels of cell branches, and length of cell branches were compared between those three areas of brain. Data were analyzed using SPSS 22 by one-way ANOVA and LSD post hoc tests. P<0.05 was considered significant. Data are presented as mean ± SD. Results: The number of microglia in the 500 µm2 of cortex (9.3± 0.5) was more than the ones in thalamus (6.3 ± 1.5, P=0.01). While the number of microglia in the 500 µm2 of hippocampus (8.3 ± 1.2) was not different with ones in cortex and thalamus (P> 0.05). Levels and length of microglia branches were not dif-ferent between cortex, hippocampus, and thalamus.Conclusion: Microglia distribution and population differences between cortex, hippocampus, and thalamus may show their importance in occurrence of neurodegenerative diseases in various area of brain. Our study did not show any difference between microstructures of branches of microglia between the cortex, hippocampus, and thalamus.

Authors

AR Afshar

The Persian Gulf Marine Biotechnology Research Center, The Per-sian Gulf Biomedical Research Institute, Bushehr, Iran

Z Farrar

The Persian Gulf Marine Biotechnology Research Center, The Per-sian Gulf Biomedical Research Institute, Bushehr, Iran

M Azmoodeh

The Persian Gulf Marine Biotechnology Research Center, The Per-sian Gulf Biomedical Research Institute, Bushehr, Iran

N Zare

The Persian Gulf Marine Biotechnology Research Center, The Per-sian Gulf Biomedical Research Institute, Bushehr, Iran